Berkeley,R.C.W., Brewer,S.J. & Ortiz,J.M. (1972). Preparation of 2-acetamido-2-deoxy-B-D-glucose oligosaccharides from acid hydrolysates of chitin by electrolytic desalting and exclusion chromatography. Anal. Biochem. 46, 687-690 Abstract

Ortiz,J.M., Berkeley,R.C.W. & Brewer,S.J. (1973). Production of exo-B-N-acetylglucosaminidase by Bacillus subtilis B. J. Gen. Microbiol. 77, 331-337 Full text

Berkeley,R.C.W., Brewer,S.J., Ortiz,J.M. & Gillespie,J.B. ( 1973 ). An exo-B-N-acetylglucosaminidase from Bacillus subtilis B: characterization. Biochem. Biophys. Acta 309, 157-168 Abstract

Brewer,S.J. & Berkeley,R.C.W. (1973). Control of the production of exo-B-N-acetylglucosaminidase by Bacillus subtilis B. Derepression during gluconeogenesis and initial stages of sporulation. Biochem.J. 134, 271-281 Full Text

Melling,J., Downs,J.D & Brewer,S.J. (1973). The effect of salts and pH on the ultrafiltration of enzymes. J.Appl. Chem Biotechnol. 23,166

Brewer,S.J., Berkeley,R.C.W. & Melling,J (1973). The partial localization of exo-B-N-acetylglucosaminidase in Bacillus subtilis B cell walls: A model based on ultrafiltration studies. Biochem. Soc. Trans. 1,1093-1095 Pay per view

Del Rio,L.A., Berkeley,R.C.W., Brewer,S.J. & Roberts S.E. (1973). An enzyme from Bacillus subtilis B with exo-B-N-acetylmuramidase activity. FEBS Letts. 37, 7-9 Full text

Brewer.S.J. & Berkeley,R.C.W. (1974). The control Bacillus subtilis B exo-B-N-acetylglucosaminidase: induction by cell wall fragments. J. Appl. Chem. Biotechnol 25, 287

Brewer.S.J. & Berkeley,R.C.W. (1973). Control of the production of exo-b-N-acetylglucosaminidase by Bacillus subtilis B. Derepression during gluconeogenesis and initial stages of sporulation. Biochem. J. 134 (271–281) Full Text

Brewer,S.J., Boyle,T.T. & Turner M.K. (1977). The carbamoylation of the 3-hydroxymethyl group of desacetyl-7-methoxycephalosporin C by homogenates of Streptomyces clavuligerus. Biochem. Soc. Trans. 5, 1026-1029 Pay per view

Brewer S.J., Farthing,J.F. & Turner,M.K. (1977). The oxygenation of the 3-methyl group of desacetoxycephalosporin by extracts of Acremonium chrysogenum. Biochem. Soc. Trans., 5, 1024-1026 Pay per view

Turner.M.K., Farthing,J.E. & Brewer,S.J. (1978). The oxygenation of 3-methyl-desacetoxycephalosporin C to 3-hydroxymethyl cephalosporin C by a 2-oxoglutarate-linked dioxygenase from Acremonium chrysogenum and streptomyces clavuligerus. Biochem. J. 173, 839-850 Full text

Brewer,S.J., Taylor,P.M. & Turner,M.K. (1980). An ATP-dependent carbamoylphosphate: 3-hydroxymethylcephem O-carbamoyl transferase from Streptomyces clavuligerus. Biochem. J. 185, 555-564 Full Text

Fleming,I.D., Nisbet,L.J. & Brewer,S.J. (1982). Target-directed antimicrobial screens. In Bioactive microbial products: Search and Discovery (Bu'Lock,J.D. ed.), Academic Press, London. pp 107-130 PDF download

Sassenfeld,H.M. & Brewer,S.J. (1984). A polypeptide fusion designed for the purification of recombinant proteins. Nature Biotechnology 2, 76-81 Abstract

Smith . J . C ., Derbyshire, R . B ., Cook, E ., Dunthorne L, Viney, J .,  Brewer,S.J., Sassenfeld,H.M. & Bell,L.D (1984). Chemical synthesis and cloning of a poly(arginine)-coding gene fragment designed to aid polypeptide purification. Gene 32, 321-327 Abstract

Brewer,S.J. & Sassenfeld,H.M. (1985). The purification of recombinant proteins using C-terminal polyarginine fusions. Trends in Biotechnol. 3, 119-122 Full text

Brewer,S.J. (1986) Quality control of proteins produced by recombinant DNA technology. J.Chem.Tech.Biotech 37,367-373 Abstract

Brewer,S.J., Dickerson,C.H., Ewbank,J & Fallon,A. (1986). Large scale high-performance liquid chromatography of urogastrone produced by recombinant DNA technology. J. Chromatog. 362, 443-449 Abstract

Brewer,S.J. & Larsen,B.R. (1987) Isolation and purification of proteins using preparative HPLC. In "Separations for Biotechnology" (Verrall,M.S. & Hudson,M.S. eds) Elis Horwood, Chichester, U.K. pp 113-126

Brewer,S.J. & Sassenfeld,H.M. (1990) Engineering proteins for purification. In "Protein Purification Applications, a Practical Approach" (Harris,E.L.V. & Angel,S. eds) IRL Press Oxford, England pp 91-112 full text

Brewer,S.J., Haymore,B.L. Hopp,T.P. & Sassenfeld,H.M. (1991).  Engineering proteins to enable their isolation in a biologically active form In "Purification and Analysis of Recombinant Proteins" (Seetharam,R. & Sharma,S.K. eds) Marcel Deeker Inc. New York, USA. pp 239-266

Miller,J.S. & Brewer,S.J. (1992). The discovery of medicines and forest conservation. In “Conservation of Plant Genes” (Adams,P.A. & Adams,J.E. eds) Academic Press, London. pp 119-134 Full text pdf

Brewer, S.J. (1994) Search for Bioactive Compounds.  In “The use of Biodiversity for Sustainable Development.” (Ed. Peter R. Seidl ) Associaco Brasilera De Quimica, Rio de Janeiro.  pp 37-43 Full text pdf

Brewer S.J. (2000) The Relationship between Natural Products and Synthetic Chemistry in the Discovery Process. in "Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries." (Eds Wrigley S. K., Hayes M.A., Thomas R., Chrystal E.J.T. & Nicholson N.) Royal Soc Chemistry Special Publication 257. pp 59-65 Full text pdf


Fleming,I.D.,Turner,M.K. & Brewer,S.J. (Priority date 19/2/76). (3)-Carbamoyl-oxymethyl-cephalosporin production. U.S. Pat. No 4075061, date 21/2/78 Full text

Brewer,S.J. & Sassenfeld,H.M. (Priority date 3/19/82). Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase. U.S. Pat. No 4532207, date 1/30/85 Full text

Brewer,S.J. & Sassenfeld,H.M. (Priority date 5/9/85). Fused polypeptides and methods for their detection. U.S. Pat No. 4880911, date 11/14/89 Full text


Large scale high pressure liquid chromatography of recombinant DNA derived Urogastrone. Presented at the 3rd International Symposium on HPLC of Proteins, Peptides and Polynucleotides. Baltimore USA, Dec. 1985.

Purification of recombinant gamma-interferon by preparative FPLC. Presented at the Engineering Foundation Conference on Recovery of Bioproducts, Uppsala, Sweden. May 1986.

Application of recombinant DNA techniques in downstream processing. IBC International Conference on Enzyme Engineering. Cambridge, U.K. Sept. 1986.

Quality control aspects of recombinant protein production. Presented at the SCI conference on Process Control of Protein Purification, London, UK Feb. 1986.

Isolation and purification of proteins using preparative HPLC. SCI International Conference on Separations in Biotechnology, Reading U.K. Sept. 1987.

Characterization of rDNA derived proteins.  Seminar at Dept. Chemistry, University Missouri, Rolla USA, Jan 1989

Screening for bioactive chemicals, an industrial perspective.  Marine Products workshop, Vancouver Canada, April 1991.

The importance of rain forest conservation to new product discovery at Monsanto.  International Symposium Tropical Forest Medical Resources and the Conservation of Biodiversity, New York USA, Jan. 1992.

Search and discovery of plant derived bioactive chemicals at Monsanto-Searle.  Testimony of Dr S.J.Brewer to the US Congressional subcommittee on the environment and natural resources, November 9, 1993 full text

Natural products and the discovery of biologically active chemicals.  Workshop on the use of biodiversity for sustainable development, Manaus, Brazil, Nov. 1993. 

Workshop on Natural Products at University of Surrey, England, 1994

Scientific principles underlying the screening approach.  At: Society for General Microbiology, 139th Meeting, University of Bradford, UK. Jan. 1998. 

Accelerating Pre-Clinical Development.  At: Assessment Of Strategies And Techniques For Successful, Biotechnology Product Development, London, June 2003

Mastering cGMP Workshop: Biologicals Manufacturing, London, November 2005, 2pm To 5pm (Registration And Coffee From 1:30pm) November 2005

Ensuring Correct Specification Setting When Managing PTMs in Biological Manufacturing
Workshop: Post Translational Modifications, Prague, Czech Republic, November 2007


Dr Steve Brewer
Cells Biochemicals Proteins
Bioproducts Technology Consultant

Stephen J. Brewer, B.Sc. Ph.D.